|
KMID : 0903620040450050261
|
|
Journal of the Korean Society for Horticultural Science
2004 Volume.45 No. 5 p.261 ~ p.269
|
|
|
Morphological Characters and Molecular Markers for Selection of Campanula portenschlagiana hybrids
|
|
Song Cheon-Young
Lee Nam-Sook
Roh Mark S.
|
|
|
Abstract
|
|
|
|
Three selections of Campanula portenschlagiana with three limited, but diverse genetic resources with regard to flower size, arrangement of inflorescences, stem length, and growth habits, were collected: a clonal selection ‘Jairai’ from Japan (CPJ) and another clone from Colorado (CPC), and two selected seedlings from Germany (CPG). These four selections were crossed to select seedlings with large flowers, compact and full stature, or with pendulous growth characters based on the analyses of morphological characters and molecular markers. Morphological characters employing 15 characters and molecular markers generated by random amplified polymorphic DNA and inter-simple sequence repeat were used to aid selection processes. In general, quantifiable characters such as flower size and stem length were inherited, respectively, from CPC and CPG. The flower type of seedlings differed greatly, showing CPC type flowers in a CPC × CPG-B 10 seedling or CPG-B flower types in CPC × CPG-B 18. Most of the hybrids except CPG-B × CPC 8 and CPC × CPG-B 18 formed inflorescences that covered the entire leaf canopy as with CPJ. According to principal coordinate analysis, stem habits, flower colors, and qualitative descriptors were mostly responsible for the variance of PRIN 1, whereas length of corollas, flower sizes, and quantifiable characters were mostly responsible for the variance of PRIN 2. Thus, these morphological characters could be used for the selection of compactness and flower sizes in hybrid populations. Parents CPC and CPJ were clustered together, but somewhat distantly clustered with seedlings that produced large flowers (seedlings of CPC × CPG-B 26, CPJ × CPC, and CPC × CPG-B 12). A semi-partial R-squared cluster analysis based on the morphological characters and a two-dimensional plot based on molecular markers showed that CPC was clustered with seedlings with large flowers. However, seedlings with large flowers, compactness, and trailing characters could be selected effectively based on the limited number of molecular markers examined in this study.
|
|
|
KEYWORD
|
|
|
inter-simple sequence repeat, random amplified polymorphic DNA, selection
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|
|